Plasmid

Part:BBa_K4840009:Design

Designed by: dty   Group: iGEM23_Jiangsu-United   (2023-09-18)


RNAi-Ready pSIREN-RetroQ-ShRNA-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3176
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1567
    Illegal BamHI site found at 1825
    Illegal BamHI site found at 6435
    Illegal XhoI site found at 3027
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1959
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 623
    Illegal BsaI site found at 644
    Illegal BsaI site found at 3505
    Illegal BsaI site found at 3526
    Illegal BsaI.rc site found at 710
    Illegal BsaI.rc site found at 1311
    Illegal BsaI.rc site found at 2846
    Illegal BsaI.rc site found at 3593
    Illegal BsaI.rc site found at 5358
    Illegal SapI site found at 4275


Design Notes

NO


Source

NO

References